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manganese
#1
This is interesting not only because manganese is radioprotective, but it supports the idea that radiation damage is a metabolic phenomenon

'manganous ions (Mn2+)
Across the tree of life, radiation resistance is governed by antioxidant Mn2+, gauged by paramagnetic resonance.
Despite concerted functional genomic efforts to understand the complex phenotype of ionizing radiation (IR) resistance, a genome sequence cannot predict whether a cell is IR-resistant or not. Instead, we report that absorption-display electron paramagnetic resonance (EPR) spectroscopy of nonirradiated cells is highly diagnostic of IR survival and repair efficiency of DNA double-strand breaks (DSBs) caused by exposure to gamma radiation across archaea, bacteria, and eukaryotes, including fungi and human cells. IR-resistant cells, which are efficient at DSB repair, contain a high cellular content of manganous ions (Mn2+) in high-symmetry (H) antioxidant complexes with small metabolites (e.g., orthophosphate, peptides), which exhibit narrow EPR signals (small zero-field splitting). In contrast, Mn2+ ions in IR-sensitive cells, which are inefficient at DSB repair, exist largely as low-symmetry (L) complexes with substantially broadened spectra seen with enzymes and strongly chelating ligands. The fraction of cellular Mn2+ present as H-complexes (H-Mn2+), as measured by EPR of live, nonirradiated Mn-replete cells, is now the strongest known gauge of biological IR resistance between and within organisms representing all three domains of life: Antioxidant H-Mn2+ complexes, not antioxidant enzymes (e.g., Mn superoxide dismutase), govern IR survival. As the pool of intracellular metabolites needed to form H-Mn2+ complexes depends on the nutritional status of the cell, we conclude that IR resistance is predominantly a metabolic phenomenon. In a cross-kingdom analysis, the vast differences in taxonomic classification, genome size, and radioresistance between cell types studied here support that IR resistance is not controlled by the repertoire of DNA repair and antioxidant enzymes.'

https://www.ncbi.nlm.nih.gov/pubmed/29042516
 
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#2
Would the manganese in mineral supplements be the right type and enough? A common supplement lists manganese sulfate 2 mg.
"The map is not the territory that it is a map of ... the word is not the thing being referred to."
 
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#3
(12-14-2017, 03:45 AM)Horse Wrote: Would the manganese in mineral supplements be the right type and enough?  A common supplement lists manganese sulfate 2 mg.

I dont know. We need a chemist. The protective complex seems to be small protein/phosphoric acid manganese complex.  There seems to be some indication you can just mix the ingredients.  One recipe might be DMSO manganese and phosphate.   "Mn2+, phosphate buffer, uridine and dimethyl sulfoxide (DMSO) increased the radioprotection of E coli 10,000 times." Maybe theres a product available?

Dont miss the message that destroys the old dogma of DNA direct hits. Protect the cell proteins, not the DNA.

'Most IR (ionizing radiation) toxicity models rank the genome as the most important cellular target. However, recent studies have shown that proteins, but not DNA, in the extremely radiation-resistant bacterium Deinococcus radiodurans are highly resistant to oxidation due to the accumulation of small-molecule manganous (Mn2+) antioxidants. In D. radiodurans, the intracellular antioxidant complexes consist mainly of Mn2+, orthophosphate (Pi) and peptides, which can functionally substitute enzymes such as superoxide dismutase and catalase .'

'D. radiodurans ultrafiltrates were found to be enriched in peptides containing aspartic acid, glutamic acid , histidine , glycine , alanine  and methionine , but not proline . This led to the synthesis of a rationally-designed decapeptide DEHGTAVMLK (decapeptide 1, DP1), yielding a complex named MDP, which forms spontaneously when DP1 (3 mM), MnCl2 (1 mM) and Pi (25 mM potassium phosphate buffer (pH 7.4)) are combined '

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'peptides are a compound consisting of two or more amino acids linked in a chain, the carboxyl group of each acid being joined to the amino group of the next by a bond of the type -OC-NH-.'

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'ortho phosphoric acid H3PO4 is prepared by adding sulfuric acid to tricalcium phosphate rock, typically found in nature as apatite. Phosphoric acid is also an ingredient in over-the-counter anti-nausea medications that also contain high levels of sugar (glucose and fructose). This acid is also used in many teeth whiteners to eliminate plaque that may be on the teeth before application.'

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MDP: A Deinococcus Mn2+-Decapeptide Complex Protects Mice from Ionizing Radiation
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976947/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933237/

(12-14-2017, 04:55 AM)Code Wrote:
(12-14-2017, 03:45 AM)Horse Wrote: Would the manganese in mineral supplements be the right type and enough?  A common supplement lists manganese sulfate 2 mg.

=========
MDP: A Deinococcus Mn2+-Decapeptide Complex Protects Mice from Ionizing Radiation
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976947/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933237/

pumpkin seeds are very high in phosphorus and manganese. MSM is converted back and forth into DMSO in the body.  I would think pumpkin seeds and MSM could be a natural approach
 
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#4
Studies have shown that manganese confers huge levels of radioprotection across different species, most notable in the bacterium radiodurans which can endure high radiation. A study showed that manganese and amino acids preserved the activity of T4 DNA ligase exposed to 60,000 Gy (6,000,000 rad?) In vivo, and protected  mice from acute radiation syndrome. All irradiated mice treated with the manganese amino complex survived exposure to 9.5 Gy in comparison to untreated mice, which displayed 63% lethality after 30 days.


"D. radiodurans has taught us – if you want to survive radiation: protect your proteins,"  (and dont worry so much about your DNA)

The nutritional story of minerals is about balance. Change one mineral level and others are affected.

'The absorption of manganese takes place in the small intestine. Manganese is then bound to the blood proteins transferrin and transmanganin and transported via the bloodstream to the body’s tissues.
When intake is low, the absorption efficiency increases, and when intake is high, the absorption efficiency decreases. This mechanism helps to maintain manganese level homeostasis in the body.'

'Some of the substances that enhance manganese absorption are histidine and citrate. Alcohol is also believed to enhance manganese absorption. On the other hand, the phytic acid and oxalic acid in many grain products and vegetables inhibit absorption.'

A high manganese intake could create problems with the absorption of certain other nutrients. Conversely, consuming any of these other minerals in large amounts could interfere with the absorption of manganese. Important minerals falling into this category include nonheme iron, magnesium, copper, zinc, calcium,  and phosphorus.

'If it is  necessary to take manganese supplements, in order to facilitate maximum absorption, they should be taken alone, without any other mineral supplements, and between meals. '

http://nutritionfactors.com/factor/detai...absorption

Is manganese safer from food than water?
 'studies have shown that children exposed to high levels of manganese through drinking water experience cognitive and behavioral deficits. 10-year old children, who were exposed to a mean manganese water concentration of 0.8 mg/L, found that children exposed to higher manganese levels had significantly lower scores on three tests of intellectual function.'
'Manganese toxicity resulting from foods alone has not been reported in humans, even though certain vegetarian diets could provide up to 20 mg/day of manganese. A study of supplementation for joint health showed a dose of elemental manganese  40 mg/day for six months gave no adverse effects.'

tolerable upper intake levels are given here http://lpi.oregonstate.edu/mic/minerals/manganese
2 mg/day for children, 11 mg/day for adults

The amount of manganese in pumpkin seeds is quite high;  4.49 mg per 100 g

Digestion of proteins and minerals is completed in the small intestine. Protein it is broken down into single amino acids, dipeptides, and tripeptides in the small intestine. They are transported through the intestine (enterocytes) by  sodium and hydrogen.  I dont see the manganese amino complexes used in the studies which showed huge radioprotection as getting through intact. Other than supplying amino acids and perhaps altering absorption to some degree, Its hard to see why a special complex is required to see the radioprotective effect of manganese.

Varioius amino acid/ manganese combinations were tested with these aminos showing best radioprotective effect

histadine
aspartic acid
glutamic acid
glycine
threonine
alanine
valine
methionine
leucine
lysine
hydroxide

bee pollen contains about 40% protein, nearly half of which are free form amino acids. 

From the above, one might conclude that eating healthy levels of manganese...toward the upper side of recommended levels, along with a full amino protein (not too much of it), like whey or bee pollen could provide significant protection from radiation

https://www.biotechdaily.com/drug-discov...ation.html
 
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#5
TaffyCat from ENEnews said this;
'years ago I knew a guy that made supplements and for those hard to digest at a good rate without much pass through he added boron for greater bio availability. If one were to take a lower dose with boron, would that be safer?'

Im neither chemist nor nutritionist.  I am fascinated at the powerful radioprotection of manganese, astounding in the organisms that incorporate it naturally.  This has implications that overturn the dogma of DNA hits as being the mechanism of low level radiation damage. The new understanding of the metabolic effect of fallout helps show the error in the standard dose model and also shows that we can take steps to counteract radiation toxicity.

Having said that, 'hacking'  nutrition is fraught with many unknown unknowns.  

I read that boron helps absorption of manganese, calcium and vitamin B2; aids the body’s use of calcium, phosphorus, magnesium and vitamin D. It strengthens bones, helps arthritis, increases mental acuity, kills yeast


One trial showed use of boron increased calcium and magnesium retention, but reduced manganese levels. http://www.acu-cell.com/b.html

It looks like its not too difficult to get pretty high levels of manganese eating certain foods.  On the other hand, some boron looks like a good idea too.  Ive used it against morgellons!
Recommended intake is 1-3 mg/day for infants up to 25 mg/day for adults
https://draxe.com/boron-uses/
 
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#6
(12-15-2017, 01:13 PM)Code Wrote: bee pollen contains about 40% protein, nearly half of which are free form amino acids. 

From the above, one might conclude that eating healthy levels of manganese...toward the upper side of recommended levels, along with a full amino protein (not too much of it), like whey or bee pollen could provide significant protection from radiation

I had read back in the days before internet that bee pollen, honey, was radio-protective.  Didn't quite understand how; thanks for the clues.  Honey is my favorite sweetener.  Years back when they started using HFCS in soda pop I quit soda and started drinking tea and honey.  Now I worry about the amount of pesticides in honey and where the tea was grown.  So, if I eat enough manganese rich foods I won't have to chew on pumpkin seeds too?  

(12-15-2017, 01:13 PM)Code Wrote: The nutritional story of minerals is about balance.

'Some of the substances that enhance manganese absorption are histidine and citrate. Alcohol is also believed to enhance manganese absorption. On the other hand, the phytic acid and oxalic acid in many grain products and vegetables inhibit absorption.'

Another reference that grain products interfere with a body's processes.  I've been avoiding bread since they started using bromides.
"The map is not the territory that it is a map of ... the word is not the thing being referred to."
 
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#7
lol... I love the taste of pumpkin seeds, feels addictive. When I have it, I add bee pollen on top of cereal, or yogurt, and even tofu, though I haven't done tofu in a long time. For those of you who garden, get your hands on the Dino Kale. It's a perennial and prolific.
Pia
Jitsi chat: enfo.pia@gmail.com
 
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#8
"Of the World's Healthiest Foods, we currently rate 21 as excellent sources of manganese."  http://www.whfoods.com/genpage.php?tname...nt&dbid=77
   
Pia
Jitsi chat: enfo.pia@gmail.com
 
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#9
(12-29-2017, 07:16 AM)piajensen Wrote: "Of the World's Healthiest Foods, we currently rate 21 as excellent sources of manganese."  http://www.whfoods.com/genpage.php?tname...nt&dbid=77

and the winner is .....CLOVES!   Although two teaspoons of cloves a day would be 'one spicy meat ball' (Jim Carry in 'the mask'

Cloves have one of the highest ORAC scores...the anti oxidant power
Also a major ingredient in the Hulda Clark anti parasite mix. By the way pumpkin seeds are also a vermifuge.
Incidentally Chitin based parasites bioaccumulate radiation. I theorize there may be a connection between nuclear fallout, cancer and fungi/parasites

"Chitin is found in invertebrates and vertebrates, and is one of the most abundant polysaccharides in nature. In addition to being directly involved in altered molting and remodeling of body plans in arthropods, the chitin-regulatory pathway plays a role in conserved processes such as cell growth, turnover, and remodeling, which are ubiquitous in all organisms and relevant to, for example, tumor formation and growth. Chitin and chitin-like proteins are also central features of both invertebrate and vertebrate immune systems."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892373/
 
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#10
cloves are high in manganese, have a high ORAC antioxidant score and have been shown to inhibit cancer growth.

Research shows that clove extract halted the activity and growth of  colon cancer , breast cancer, ovarian cancer ,liver cancer and  colon cancer cells. Meanwhile, the clove extract was not dangerous to  normal cells.



https://www.ncbi.nlm.nih.gov/pubmed/22292639

https://www.ncbi.nlm.nih.gov/pubmed/28524540





Pumpkin seeds 

studies show pumpkin seed extract eliminated cancer cells by inducing apoptosis.
 Antitumor effects of cucurmosin in human chronic myeloid leukemia occur through cell cycle arrest and decrease the Bcl-2/Bax ratio to induce apoptosis,  determined the apoptosis effect of pumpkin seed on leukemia cancer cells 
https://www.ncbi.nlm.nih.gov/pubmed/21964700
https://www.ncbi.nlm.nih.gov/pubmed/23157594/

Recently there have been many articles on the dangers of lectins. Lectins are plant protective chemicals that reduce nutritional absorption and can cause inflammation and leaky gut syndrome.  All foods contain various lectins, so they are not avoidable.  But seeds, nuts bean and grains  have higher levels.  One can reduce the levels by soaking overnight and by pressure cooking.  Of course other nutrients are lost by cooking.   The manganese bioavailability may increase if pumpkin seeds are soaked.



I will reiterate that the ability of minerals and herbs to protect against cancer demonstrates that cancer is a metabolic disease. Low level nuclear fallout causes a cascade of metabolic changes that are more important in causing disease than the DNA hit model. This helps explain why levels of radiation from fallout that are equal to background radiation is so dangerous.  
 
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#11
(01-08-2018, 02:29 PM)Code Wrote: I will reiterate that the ability of minerals and herbs to protect against cancer demonstrates that cancer is a metabolic disease. Low level nuclear fallout causes a cascade of metabolic changes that are more important in causing disease than the DNA hit model. This helps explain why levels of radiation from fallout that are equal to background radiation is so dangerous. 

To be more accurate, I would have said that levels of man-made ionizing radiation from fallout that are less than background can be so dangerous.  I agree that cancer seems to be a metabolic disease.  The depletion of phytonutrients and minerals in the topsoil our food is grown in means the foods we eat may not contain as much as we need to stay healthy.  We may need to eat more fruits and vegetables to get enough of these important nutrients.  

Phytonutrients
https://www.webmd.com/diet/guide/phytonutrients-faq#1
"The map is not the territory that it is a map of ... the word is not the thing being referred to."
 
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#12
when mans poisons are turning your world gray, flood yourself with color. Purple cabbage is good.  See the intense color from hibiscus.  Hibiscus has been shown to fight cancer in studies. Propolis from the beehive.  Tumeric...
 
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#13
I'm growing purple carrots this year, I'll eat some but my plan is to let them mature and go to seed (year two) so I can grow more for eating each year. No tumeric in the stores, want a couple fingers to plant. The ginger is growing well as are a bunch of other fruit and veg plants. Found a Preying Mantis in the black seed sunflower patch the other day.
Pia
Jitsi chat: enfo.pia@gmail.com
 
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